Synthesized:Article Title: Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal
Article Snippet: .. Complementary deoxyribonucleic acid (cDNA) was synthesized using 200 ng of total RNA and SuperScript IV VILO with ezDNase kit (Thermo Fisher Scientific). ..
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
Expressing:Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
Bacteria:Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
Incubation:Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
Real-time Polymerase Chain Reaction:Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
SYBR Green Assay:Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 h as described below. cDNA was synthesized from 20 ng total RNA, prepared as described above using a SuperScript IV Vilo with ezDNase kit (Thermo) according to the manufacturer's instructions. qPCR was performed using PowerUp SYBR green (Life Technologies) mixed with 10 ng cDNA and 600 nM the primers indicated in Table S4. .. Amplification was performed on a StepOne Plus real-time PCR system (Life Technologies) using the following conditions: 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve analysis and gel electrophoresis confirmed amplification of a single product for all genes examined (data not shown).
Article Title: A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice
Article Snippet: .. For cps3S expression, bacteria were incubated in PBS for 30 min or 3 hrs as 433 described below. cDNA was synthesized from 20 ng total RNA prepared as described above 434 using the SuperScript IV VILO with ezDNase kit (Thermo) according to the manufacturer’s 435 instructions. qPCR was performed using PowerUp SYBR Green (Life Technologies) mixed with 436 10 ng cDNA and 600 nM of the primers indicated in Table S4. .. Amplification was performed on 437 a StepOne Plus Real Time PCR System (Life Technologies) using the following conditions: 438 50°C for 2 min, 95°C for 2 min, and 40 cycles of 95°C for 3 s and 60°C for 30 s. Melt curve 439 analysis and gel electrophoresis confirmed amplification of a single product for all genes 440 examined (data not shown).
cDNA Synthesis:
|